Device

Part:BBa_K119009:Design

Designed by: Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualp   Group: iGEM08_LCG-UNAM-Mexico   (2008-09-29)


cI promoter RcnR op RcnA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 677
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We needed to repress the RcnA activity, although niquel is present in the cell. In order to achieve this, we added cI promoter(BBa_R0051) (an operator) sequence which is regulated by cI.


Source

Constructed by LCG-UNAM-Mexico Team

References